潘明政,郭赛珊,梁晓春,唐代屹,张建伟,孙仁宇.糖尿病大鼠心肌糖基化终产物受体mRNA表达及仙贞片对其影响的实验研究[J].中国中西医结合杂志,2002,(10):773-775 |
糖尿病大鼠心肌糖基化终产物受体mRNA表达及仙贞片对其影响的实验研究 |
Experimental Study on mRNA Expression of Advanced Glycosylation End Product Receptor in Myocardium of Diabetic Rats and Influence of Xianzhen Tablet on It |
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DOI: |
中文关键词: 糖尿病 心肌 蛋白非酶糖基化终产物受休 |
英文关键词:Diabetes mellitus myocardium receptor of advanced glycosylationend product |
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中文摘要: |
目的 :观察仙贞片对糖尿病大鼠心肌内皮细胞蛋白非酶糖基化终产物受休mRNA(RAGE mRNA)高表达的影响。方法 :采用链脲佐菌素左下腹腔内单次注射造成糖尿病大鼠模型 ,随机分模型组、仙贞片组及氨基胍组各 10只 ,另设正常对照组 10只。应用原位杂交的方法检测RAGE mRNA表达。结果 :模型组大鼠心肌内皮细胞RAGE mRNA表达灰度值明显增高 ,出现过度表达。仙贞片治疗后心肌内皮细胞RAGE mRNA表达灰度值降低 ,与氨基胍组相近似 (P >0 0 5 ) ,与模型组比较差异显著 (P <0 0 5 )。显示仙贞片与氨基胍一样 ,可显著下调RAGE mRNA的过度表达 ,对糖尿病大鼠心肌RAGE mRNA高表达有明显调节作用。结论 :仙贞片对高血糖造成的心肌损害具有保护作用。 |
英文摘要: |
Objective: To observe the effect of Xianzhen tablet (XZT) on high expression of advanced glycosylation end product receptor (RAGE) in myocardium of diabetic rats. Methods: Rat diabetic model was made by intraperitoneal streptozotocin injection and divided into the model group, the XZT group and the positive control group (treated with aminoguanidine). And a normal control group was also set up. RAGE mRNA expression in myocardial endothelial cells of rats was detected by in situ hybridization. Results: Gray value of RAGE mRNA was increased after modeling, revealing overexpression. It lowered significantly after XZT treatment with the value similar to that after aminoguanidine treatment but significantly different from that in the model group (P<0 05), suggesting that XZT could down regulate the overexpression of RAGE mRNA. Conclusion: XZT has effect in protecting myocardium from hyperglycemia induced damage. |
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