中药金叶败毒颗粒抑制人巨细胞病毒感染丝裂素活化蛋白激酶p38信号通路的研究

Study on Effect of Jingye Baidu Granule on Inhibition of Mitosin Activating Protein Kinase (p38) Signaling Pathway with Human Cytomegalovirus Infection

免费下载全文查看/发表评论下载PDF阅读器

DOI：

中文关键词: 金叶败毒颗粒巨细胞病毒丝裂素活化蛋白激酶

英文关键词:Jinye Baidu Granule cytomegalovirus mitosin activating protein kinase

基金项目:国家“九五”重点攻关项目资助 (No .96 - 90 4 - 0 6 - 0 8)

作者	单位
汪辉	浙江大学医学院附属妇产科医院博士后流动站
闻良珍	武汉同济医科大学附属同济医院妇产科
凌霞珍	武汉同济医科大学附属同济医院妇产科

摘要点击次数: 1548

全文下载次数: 1657

中文摘要:

目的 :研究金叶败毒颗粒 (简称中药 )对人巨细胞病毒 (HCMV)感染丝裂素活化蛋白激酶(MAPK) p38信号通路的抑制作用 ,并探讨其治疗HCMV感染的分子机制。方法 :应用原位杂交和免疫组化方法分别检测中药和更昔洛韦 (GCV)干预HCMV感染的细胞 p38mRNA及 pRb蛋白表达水平 ,观察两种药物对HCMV感染人胚胎成纤维细胞 (HEL)的影响。结果 :两种药物均可抑制HCMV在HEL中的增殖 ,但中药能明显抑制 p38mRNA的水平 ,并使其磷酸化底物 pRb蛋白表达降低 ,而GCV无此作用。结论 :中药可能通过调节MAPK通路而抑制HCMV基因的表达和复制

英文摘要:

Objective: To explore the mechanism of Jinye Baidu Granule (JYBDG) in treating human cytomegalovirus (HCMV) infection by studying its inhibitory effect on mitosin activating protein kinase (MAPK) pathway with HCMV infection. Methods: Expressions of p38 and pRb were investigated by means of in situ hybridization and immunohistochemistry before and after treated by JYBDG or Ganciclovir, and the development of cytopathic effect (CPE) was also observed. Results: Both JYBDG and Ganciclovir could inhibit HCMV proliferation in HEL, but JYBDG showed obvious inhibition on p38mRNA level and lower the pRb protein expression, while Ganciclovir didn′t show. Conclusion: JYBDG could inhibit the gene expression and duplication of HCMV through regulating MAPK pathway.

关闭