苏木对同种异位心脏移植大鼠心肌穿孔素mRNA表达的影响

作者	单位
周亚滨	黑龙江中医药大学附属第一医院哈尔滨150040
姚凤祯	黑龙江中医药大学附属第一医院哈尔滨150040
韩佳瑞	黑龙江中医药大学附属第一医院哈尔滨150040
杨焱	黑龙江中医药大学附属第一医院哈尔滨150040
张春芳	黑龙江中医药大学附属第一医院哈尔滨150040
石月平	黑龙江中医药大学附属第一医院哈尔滨150040

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中文摘要:

目的 :观察苏木 (SappanWood)对同种异位心脏移植大鼠心肌穿孔素 (perforin ,PFP)mRNA表达的影响。方法 :以Wistar大鼠为供体 ,以SD大鼠为受体 ,建立腹腔异位心脏移植模型 ,以RT PCR法检测苏木对穿孔素mRNA表达的影响。结果 :苏木可明显降低穿孔素mRNA的表达 ,并能明显减轻病理形态学及心肌细胞超微结构的损伤。结论 :苏木有明显的抗免疫排斥作用 ,可能是通过降低穿孔素mRNA的表达而发挥免疫抑制的作用。

英文摘要:

Objective: To observe the effect of Sappan wood (SW) on the expression of perforin mRNA in myocardium of rats after allogeneic cardiac transplantation. Methods: The animal model of allogeneic (abdominal) cardiac transplantation was established by taking Wistar rat as provider and SD rat as receptor, perforin mRNA expression in the model′s myocardium was detected by RT PCR. Results: SW could obviously reduce the perforin mRNA expression, it also could alleviate the pathological morphology and ultrastructural damage of myocardial cells. Conclusion: SW has obvious effect in antagonizing immune rejection after transplantation, the mechanism of its immunosuppression could be through lowering the perforin mRNA expression.

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