| 董妍君,李卫东,屠呦呦,邹万忠,膝慧玲,林志彬.双氢青蒿素对BXSB狼疮小鼠自身抗体产生、TNFα分泌及狼疮性肾炎病理改变的影响[J].中国中西医结合杂志,2003,(9):676-679 |
| 双氢青蒿素对BXSB狼疮小鼠自身抗体产生、TNFα分泌及狼疮性肾炎病理改变的影响 |
| Effect of Dihydro-Qinghaosu on Auto-antibody Production, TNFα Secretion and Pathologic Change of Lupus Nephritis in BXSB Mice |
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| DOI： |
| 中文关键词: 双氢青蒿素 BXSB小鼠 抗双链DNA抗体 狼疮性肾炎 系统性红斑狼疮 肿瘤坏死因子α |
| 英文关键词:dihydro-qinghaosu BXSB mice anti-ds-DNA antibody lupus nephritis systemic lupus erythematosus tumor necrosis factor α |
| 基金项目:国家自然科学基金资助项目 (No .30 1 4 0 0 2 4 ) |
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| 中文摘要: |
| 目的 :观察双氢青蒿素 (DQHS)对BXSB狼疮模型小鼠几项免疫指标及狼疮性肾炎 (lupusnephri tis,LN)病理改变的影响 ,探讨DQHS治疗系统性红斑狼疮 (systemiclupuserythematosus,SLE)的可能机制。方法 :采用ELISA方法检测血清中的抗双链DNA(ds DNA)抗体 ,以ELISA试剂盒检测血清中肿瘤坏死因子α(TNFα)的水平 ,对肾脏组织进行HE常规染色及Masson特殊染色。结果 :血清中TNFα、抗ds DNA抗体水平 ,DQHS 12 5mg/kg给药组及DQHS 2 5mg/kg给药组与模型组比较 ,差异有显著性 (P <0 0 5或P <0 0 1) ;各组肾脏组织HE、Masson染色病理改变差异有显著性。结论 :DQHS能抑制BXSB小鼠血清抗ds DNA抗体的生成 ,对BXSB小鼠血清中TNFα的分泌有抑制作用 ,能明显改善BXSB小鼠LN的病理状态。 |
| 英文摘要: |
| Objective: To investigate the effect of dihydro-qinghaosu (DQHS) on auto-antibody production, TNFα secretion and pathologic change of lupus nephritis in BXSB mice and the possible mechanism of DQHS in treating systemic lupus erythematosus (SLE). Methods: Anti ds-DNA antibody and TNFα in serum of the BXSB mice were detected by enzyme linked immunosorbent assay (ELISA). Renal tissue was stained by HE and Masson. Results: In the hight and moderate dose DQHS groups, as compared with the model group, levels of anti-ds-DNA antibody and serum TNFα were significantly lower (P<0 05); and renal pathological change was milder. Conclusion: DQHS could inhibit the production of anti-ds-DNA antibody and secretion of TNFα and improve the pathologic lesion of lupus nephritis in BXSB mice. |
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