| 董晓先,刘金保,董燕湘,肖庆忠.天麻诱导骨髓间质干细胞分化为神经元样细胞的实验研究[J].中国中西医结合杂志,2004,(1):51-54 |
| 天麻诱导骨髓间质干细胞分化为神经元样细胞的实验研究 |
| Experimental Study on Effect of Gastrodia in Inducing the Differentiation of Mesenchymal Stem Cells into Neuron-like Cells |
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| DOI： |
| 中文关键词: 天麻 骨髓间质干细胞 神经细胞 定向分化 |
| 英文关键词:gastrodia bone marrow mesenchymal stem cell nerve cell oriented differentiation |
| 基金项目:国家 8 63计划基金资助项目 (No .2 0 0 2AA2 0 4 0 1 1 );广州市科技攻关引导项目 (No .2 0 0 3Z3 E0 0 1 2 ) |
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| 中文摘要: |
| 目的 :探讨天麻对体外定向诱导大鼠骨髓间质干细胞 (MSC)分化为神经元样细胞的影响。方法 :通过贴壁法分离大鼠MSC ,体外扩增培养。中药天麻定向诱导分化为类神经元样细胞。光镜下观察细胞形态 ,免疫细胞化学法检测神经细胞特异性抗原标志物神经原烯醇化酶 (NSE)、胶质纤维酸性蛋白(GFAP)、巢蛋白 (Nestin)。结果 :大鼠MSC可通过贴壁法成功分离并可在体外大量扩增。天麻诱导 2h后大部分可分化为神经元样细胞 ,出现胞体和突起 ,免疫细胞化学染色NSE、Nestin呈阳性 ,GFAP阴性。结论 :大鼠MSC可诱导分化为神经元样细胞。 |
| 英文摘要: |
| Objective:To investigate the effect of gastrodia in extracorporeal oriented inducing the differentiatin of mesenchymal stem cells into neuron-like cells. Methods:Mesenchymal stem cells were separated from bone marrow of rats by wall sticking method, amplifying cultured in vitro, and differentiated into neuron-like cells by oriented induction with gastrodia. The morphology of cells was observed under light microscopy, neuro-specific enolase (NSE), nestin and glial fibrillary acidic protein (GFAP) were detected by immunocytochemistry. Results:Rats mesenchymal stem cells could be separated and amplified in vitro. After being induced by gastrodia for 2 hrs, most of the cells wouldbe differentiated into meuron-like cells, revealing cytodendrite. By immunochemical staining, cells showed positive of NSE, nestin, and negative of GFAP. Conclusion:Rats′ mesenchymal stem cells could be induced to differentiate into neuron-like cells. |
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