| 王玉东,李大金,朱影.补肾宁心方对小鼠成骨细胞的增殖和抗凋亡作用[J].中国中西医结合杂志,2004,(3):230-233 |
| 补肾宁心方对小鼠成骨细胞的增殖和抗凋亡作用 |
| Effect of Bushen Ningxin Decoction on Proliferation and Apoptosis of Murine Osteoblast |
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| DOI： |
| 中文关键词: 补肾宁心方 成骨细胞 增殖 凋亡 |
| 英文关键词:Bushen Ningxin decoction osteoblasts proliferation apoptosis |
| 基金项目:上海市科技攻关项目(No.004019061) |
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| 中文摘要: |
| 目的 探讨补肾宁心方促进成骨细胞增殖和抗凋亡作用。方法 建立BALB/c小鼠绝经后骨质疏松模型,给予中药补肾宁心方浓缩液(简称中药组)灌服12周,灌服雌激素为阳性对照组(简称E2组),灌服生理盐水为阴性对照组(简称对照组)。12周末处死各组动物取颅骨,以植块法培养成骨细胞,MTT法测其增殖能力;取股骨,以透射电镜观察其细胞器的变化;取药物血清体外观察其抗成骨细胞凋亡作用。结果中药组和E2组细胞器丰富,高尔基体发达,胶原纤维致密;中药组比对照组来源的成骨细胞具有更强的增殖能力(P<0.05);10％-20％的中药药物血清在体外能有效抑制成骨细胞的凋亡。结论 中药补肾宁心方具有促成骨细胞增殖和抗凋亡作用。 |
| 英文摘要: |
| To investigate the effect of Bushen Ningxin decoction (BSNXD) on postmenopausal osteoporosis. Methods Postmenopausal osteoporosis model of female BALB/c mice were founded and afforded with BSNXD. The experiment was ceased, and the skeleton and blood of mice in each group were collected following 12 weeks of the treatment. Change of osteoblastic organelle in right femur was observed by transmission electron microscope (TEM). Osteoblasts from calvaria of different groups were isolated and cultured by the tissue fragment migrating growth method and the proliferation ratio of osteoblasts in the second passage was examined with thiazolyl blue reagents when cultured for 1 day and 5 days, respectively. Results TEM showed that the osteoblasts of the animal treated by BSNXD had more cell organelles and developed Golgi complex, whose ability of proliferation(P<0.05)and anti-apoptosis is stronger than that of OVX group. Conclusion BSNXD can accelerate the proliferation and inhibit the apoptosis of osteoblasts, the effective concentration of pharmacological serum varies from 10% to 20% . |
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