动脉粥样硬化大鼠“痰瘀”病理演变与VSMC肌动蛋白表达的相关性研究

Relationship between Pathological Development of Phlegm-Stasis and Expression of Actin in Vascular Smooth Muscle Cell in Atherosclerosis Rat Model

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中文关键词: 动脉粥样硬化动物模型血管平滑肌细胞肌动蛋白表达

英文关键词:atherosclerosis animal model vascular smooth muscle cell actin expression

基金项目:湖南省教育厅重点资助项目(No.99A03);湖南省卫生厅资助项目(No.200215);中国博士后科学基金项目(No.2003033474)

作者	单位
王东生	中南大学湘雅医院血液科长沙 410008
袁肇凯	中南大学湘雅医院血液科长沙 410008
陈方平	中南大学湘雅医院血液科长沙 410008
黄献平	中南大学湘雅医院血液科长沙 410008

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中文摘要:

目的探讨动脉粥样硬化模型大鼠随着造模时间的推移“痰瘀”演变规律。方法采用高脂饲料喂饲法复制大鼠动脉粥样硬化模型,用生化法及免疫组化方法动态检测血脂、血液流变学、血糖、胰岛素及血管平滑肌细胞(VSMC)肌动蛋白表达的变化。结果随着动脉粥样硬化大鼠由痰到瘀症状的变化(即血脂、血液流变学、血糖、胰岛素及胰岛素敏感指数等指标均随病情加重而变化),VSMC肌动蛋白表达逐渐增加。结论 VSMC肌动蛋白表达是动脉粥样硬化大鼠由痰到瘀的分子机制。

英文摘要:

To explore the dynamic change of Phlegm-stasis in the rat atherosclerotic model as the time goes on. Methods Adopting high fat forage fed to develop the atherosclerotic model in rats, and the changes of blood lipid, hemorrheology, blood glucose, insulin and vascular smooth muscle cell (VSMC) actin expression were detected by biochemical and immunohistochemical assay at various time points after modeling. Results The expression of VSMC actin gradually increased along with the change of model rats’ Syndrome from Phlegm to stasis, i.e. , the change of parameters, including blood lipid, hemorrheologic parameters, blood glucose, insulin and insulin sensitive indexes along with the aggravation of disease. Conclusion The expression of VSMC actin could be the molecular mechanism for the Syndrome developing from Phlegm to stasis in atherosclerotic rats.

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