冰片对血脑屏障体外模型细胞间紧密连接和细胞吞饮囊泡的影响

目的探讨冰片开放血脑屏障的机制。方法制备含冰片的家兔血清 ,应用马丁达比狗肾上皮细胞系作为血脑屏障的体外模型 ,观察冰片对血脑屏障细胞间紧密连接、细胞吞饮囊泡的影响。结果冰片能使血脑屏障体外模型细胞间紧密连接结构减少、细胞吞饮囊泡数量增加、粒径增大 ,冰片血清处理 4h后先打开细胞间紧密连接 ,处理 2 4h后才对细胞吞饮造成影响 ;移除冰片血清 2 4h后上述影响即消失 ,说明上述作用具有可逆性。结论冰片能明显使血脑屏障细胞间紧密连接松散 ,使物质经细胞间通道转运加速 ;冰片能明显使血脑屏障细胞吞饮小泡数量增多、体积增大 ,从而使经细胞吞饮的物质转运加速

英文摘要:

ObjectiveTo explore the mechanism of borneol in opening the blood-brain barrier (BBB). MethodsBorneol contained serum was prepared and using Matin-Darby canine kidney epithelium (MDCKE) cell line as the in vitro BBB model to observe the effects of borneol on intercellular tight junction (ICTJ) and pinocytosis vesicles of BBB model. ResultsBorneol reduced the ICTJ and caused increase of the number and enlarged the diameter of vesicles. The ICTJ was opened firstly 4 hrs after borneol treatment, then the pinocytosis was affected 24 hrs later. The effects disappeared 24 hrs after removal of the borneol contained serum, indicating that the above-mentioned effects were reversible. ConclusionBorneol could obviously loosen the ICTJ in BBB, accelerate the transportation of substance through the intercellular passage, it also could increase the number and volume of pinocytosis vesicles in BBB cells, thus to accelerate the transportation of substance by way of cell pinocytosis.

关闭