加味木防己汤抗大鼠实验性关节炎的研究

目的探讨加味木防己汤对大鼠实验性佐剂关节炎(adjuvantarthritis,AA)的作用及其机制。方法应用弗氏完全佐剂注射诱发大鼠产生实验性关节炎,第18天开始给予加味木防己汤灌胃,39天测量AA大鼠体重,脾及胸腺指数,足肿胀度。HE染色观察大鼠踝关节组织病理学变化,ELISA法测定大鼠血清中白细胞介素-1β(IL-1β),肿瘤坏死因子-α(TNF-α)的含量。结果加味木防己汤能缓解AA病情,使足肿胀度下降,体重及脏器指数改善,关节水肿减轻,淋巴细胞浸润明显减少,滑膜增生受抑制。血清IL-1β,TNF-α的含量明显下降。结论加味木防己汤对AA大鼠的治疗作用,可能与其下调IL-1β,TNF-α的含量有关。

英文摘要:

ObjectiveTo explore the therapeutic effect of Jiawu Mufangji Decoction (JMD) in treating rats with adjuvant arthritis (AA) and its mechanism. MethodsAA model rats induced by Freund’s complete adjuvant were treated with JMD by gastrogavage starting from 18 days after modeling. On the 39th day, body weight, spleen and thymus index, and swelling degree of paw of the AA rats were measured, pathological changes of the ankle joint tissue were observed using HE staining, and serum levels of interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) were determined by enzyme-linked immunosorbent assay (ELISA). ResultsJMD could relieve the symptoms of AA rats, decrease the paw swelling, improve the weight and spleen and thymus index, reduce the dropsy of joints and lymphocytes infiltration, inhibit the proliferation of synovium, and obviously lower the serum levels of interleukin-1β and TNF-α. ConclusionThe therapeutic effect of JMD might be related to its action in down-regulating the serum levels of IL-1β and tumor necrosis factor α.

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