紫草油对创面组织修复及bFGF mRNA表达的影响

作者	单位
裴宪武	西安交通大学第二医院骨科
王坤正	浙江省台州市中心医院骨科
陈君长	浙江省台州市中心医院骨科
党晓谦	浙江省台州市中心医院骨科
时志斌	浙江省台州市中心医院骨科
高登峰	浙江省台州市中心医院骨科

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中文摘要:

目的通过观察创面组织碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)mRNA的表达和创面愈合率,探讨紫草油促进创面愈合的分子生物学机制。方法对住院患者的皮肤创面用紫草油治疗为观察组,对照组用凡士林纱布治疗,观察两组的创面愈合率。用RT-PCR方法观察两组创面组织的bFGF mRNA表达。结果两组创面组织均存在内源性bFGF mRNA基因表达,观察组创面组织bFGF基因表达在各个时段均明显超过对照组,灰密度值比较,差异有显著性(P<0·05);创面愈合率与创面组织中bFGF mRNA基因表达程度相平行,而观察组的创面愈合率明显高于对照组(P<0·05)。在创面愈合的不同阶段中,作为内参的磷酸甘油醛脱氢酶(GAPDH)基因都有表达,并且各时段GAPDH基因表达量无显著变化(P>0·05)。结论紫草油对创面组织中bFGF有明显的促进作用,这可能是紫草油提高创面愈合率的机理之一。

英文摘要:

ObjectiveTo explore the molecular biological mechanism of Arnebia Root oil (AO) in promoting the recovery of surface of wound by observing basic fibroblast growth factor (bFGF) mRNA expression in the wound tissue and healing rate of the wound. MethodsPatients in the observed group with their wound treated by AO and those in the control group treated by petrolatum gauze. The wound surface healing rate was estimated and bFGF mRNA expression was observed by RT-PCR. ResultsEndogenous bFGF mRNA expression existed in the wound surface of both groups, but its level in the observed group at any time point was obviously higher than that in the control group respectively, with significant difference in comparison of the gray density between the two groups (P<0.05). The wound surface healing rate kept abreast with bFGF mRNA expression in wound tissues, so it was significantly higher in the observed group than that in the control group (P<0.05). GAPDH gene, which was taken as a parameter for internal reference, expressed with a certain amount unchanged in different periods of healing (P>0.05). ConclusionAO shows obviously promotive action on bFGF, an important regulatory factor on wound healing, it might complete the recovery process by stimulating the increase of bFGF.

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