芪参益气滴丸干预糖尿病大鼠虹膜微血管病变的作用机制研究

目的采用激光共聚焦显微镜超速摄像技术和荧光白蛋白标记技术,动态观察芪参益气滴丸干预糖尿病大鼠虹膜微血管病变的作用机制。方法链脲佐菌素(STZ)腹腔注射建立1型糖尿病大鼠模型,随机分为模型组、治疗组、对照组,并设正常组。治疗组灌服芪参益气药液,对照组灌服多贝斯液[1g/(kg.d)],疗程10个月。利用激光共聚焦显微镜超速摄像动态观察虹膜微循环状态。结果治疗组虹膜血流速度明显加快,虹膜血管内、外荧光密度降低,荧光渗漏面积及虹膜血管管径明显缩小,与模型组比较,差异均有统计学意义(P<0.01)。结论芪参益气滴丸在改善虹膜微血管流态方面的确有一定作用,可以促进血流加快,抑制血管异常扩张及改善虹膜微血管的通透性异常增高状态。

英文摘要:

Objective Use laser confocal microscopy overspeed camera technique and fluorescence albumin labeling to study the acting mechanism of Qishen Yiqi Drop Pill (QYDP) for intervening irido-microangiopathy (IMAP) in diabetic rats. Methods Rat model of diabetes mellitus type 1 (DM1) was established by intraperitoneal injection of streptozocin (STZ). The model rats were randomly divided into three groups, the treatment group, the model group and the control group. At the same time a normal control group was set up. The treatment group was medicated with QYDP (prepared into liquid form), and the control group with Duobeisi liquor (1g/kg per day) for 10 months. The dynamic state of iris microcirculation in rats was observed using laser confocal microscopy overspeed camera. Results Compared with the treatment group, blood flow in iris of model rats was slower significantly (P<0.01); the fluorescence density and leakage area of inside and outside iris vessels, and the iris vascular diameter were significantly higher in the model group than those in the treatment group (P<0.01). Conclusion QYDP has definite effect in improving iris microcirculation, which can accelerate the blood flow, inhibit the abnormal expansion of vessels and improve the increased iris micro-vascular permeability.

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