急性肝衰竭模型中医证型探讨

目的应用小鼠急性肝衰竭模型,筛选有效方剂,以方测证探讨该模型的中医证型。方法采用腹腔注射脂多糖(lipopolysaccharides,LPS)/D-氨基半乳糖胺(D-galactosamine,D-GalN)建立小鼠急性肝衰竭模型,给予茵陈蒿汤、黄连解毒汤、补中益气汤、犀角地黄汤灌胃干预。观察动物的行为及生存率,检测肝功能,评价肝组织病理损伤。结果所测经典方剂中,犀角地黄汤可使小鼠急性肝衰竭模型的生存率由10%提高到60%。造模5h后,犀角地黄汤组ALT为(183.95±52.00)U/L、AST为(235.70±34.03)U/L低于模型对照组[ALT:(213.32±71.93)U/L;AST:(299.48±70.56)U/L],但差异无统计学意义(P>0.05);犀角地黄汤可以明显改善肝脏的损伤。结论犀角地黄汤是治疗LPS/D-GalN急性肝衰竭模型的有效方剂,按照以方测证原则,LPS/D-GalN小鼠急性肝衰竭模型以热毒血瘀证为主。

英文摘要:

Objective To study Chinese syndrome typing of acute hepatic failure (AHF) mice model by screening effective formulae. Methods Lipoplysaccharides (LPS)/D-galactosamine (D-GalN) was intraperitoneally injected to mice to establish the AHF mice model. Yinchenhao Decoction,Huanglian Jiedu Decoction,Buzhong Yiqi Decoction,and Xijiao Dihuang Decoction were administered to model mice respectively by gastrogavage. The behavior and the survival rate were monitored. The liver function and pathological changes of liver tissues were detected. Results In all the tested classic recipes,the survival rate was elevated from 10% to 60% by administration of Xijiao Dihuang Decoction. Five h after modeling,the serum alanine aminotransferase (ALT) level was (183.95±52.00) U/L,and aspartate aminotransferase (AST)(235.70±34.03) U/L in Xijiao Dihuang Decoction Group,lower than those of the model control group,but with insignificant difference (ALT:213.32±71.93 U/L; AST:299.48±70.56 U/L,both P>0.05). Xijiao Dihuang Decoction could obviously alleviate the liver injury. Conclusions Xijiao Dihuang Decoction was an effective formula for LPS/D-GalN induced AHF model. According to syndrome typing through formula effect,heat toxin and blood stasis syndrome dominated in the LPS/D-GalN induced AHF mice model.

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