五味子多糖对环磷酰胺致生精障碍大鼠的治疗作用及对生殖激素的影响

目的探讨五味子多糖对环磷酰胺（CTX）致生精障碍大鼠的治疗作用及其对生殖激素的影响。方法醇碱法提取五味子多糖。50只雄性Wistar大鼠随机分为正常组、模型组以及五味子多糖低、中、高剂量组。除正常组外，其余4组均给予腹腔注射CTX 80 mg/（kg·d）连续5天，制备生精障碍大鼠模型。造模后，五味子多糖低、中、高剂量组分别给予五味子多糖100、200、400 mg/（kg·d）灌胃，正常组和模型组给予等体积生理盐水灌胃，均每天1次，连续60天。末次灌胃后24 h，取大鼠血清和睾丸组织。酶联免疫法测定大鼠血清卵泡刺激素（FSH）、黄体生成素（LH）和睾丸组织匀浆睾酮（T）水平；比较各组大鼠精子密度、活率及精子畸形率；HE染色观察睾丸组织形态。结果与正常组比较，模型组精子密度和精子活率降低，精子畸形率升高，血清FSH和LH水平升高，睾丸组织匀浆中T含量降低，差异均有统计学意义（P<0.01）。与模型组比较，五味子多糖各剂量组精子密度、精子活率升高，精子畸形率降低，血清FSH和LH水平降低，睾丸组织匀浆中T含量升高，差异均有统计学意义（P<0.01，P<0.05），且五味子多糖各剂量组呈剂量依赖性。组织学观察显示，五味子多糖各剂量组大鼠睾丸组织病理性损害均得到改善。结论五味子多糖对CTX所致大鼠生精障碍具有明显的治疗作用，其机制可能与恢复下丘脑—垂体—性腺轴的调控功能有关。

英文摘要:

Objective To explore the efficacy of Schizandra Chinensis polysaccharide （SCP） on cyclophosphamide （CTX） induced dyszoospermia of rats and its effects on reproductive hormones. Methods SCP was extracted by ethanol alkali solution. Fifty male Wistar rats were randomly divided into 5 groups，i.e.， the normal control group， the model group， the low dose SCP group （100 mg/kg）， the middle dose SCP group （200 mg/kg）， and the high dose SCP group （400 mg/kg）. Except the normal control group， the dyszoospermia rat model was established by peritoneal injection of CTX at the daily dose of 80 mg/kg， once daily for 5 successive days. After modeling， SCP was intragastrically administered at corresponding dose to the three SCP groups. Equal volume of normal saline was given to rats in the normal control group and the model group by gastrogavage. All the medication was performed once daily for 60 successive days. The blood serum and testis were withdrawal 24 h after the last intragastric administration. The levels of follicle stimulating hormone （FSH）， luteinizing hormone （LH）， and testosterone （T） in the testis homogenate were determined by enzyme linked immunosorbent assay. The sperm count， the motility rate， and the teratospermia rate were compared. The morphology of the testis was observed using HE staining. Results Compared with the normal control group， the sperm count and the motility rate decreased， the teratospermia rate increased， the serum levels of FSH and LH increased， the T content in the testis homogenate decreased in the model group， showing statistical difference （P<0.01）. Compared with the model group， the sperm count and the motility rate increased， the teratospermia rate decreased， the serum levels of FSH and LH decreased， the T content in the testis homogenate increased in the model group， showing statistical difference （P<0.01， P<0.05）. All the indices showed dose dependent manner in the SCP groups. The histological results showed the pathological injury in the testicular tissue was improved in all SCP groups. Conclusion SCP showed obvious therapeutical effects on CTX induced dyszoospermia in rats， and its mechanisms might be correlate with recovering the regulation function of hypothalamus hypophysis gonad axis.

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